Semi-automated biofoundry workflows for sequence coevolution-guided isoprene synthase engineering

We developed biofoundry workflows for efficient enzyme engineering, integrating a simple and accessible computational tool with automation to reduce time and resources. Using this approach, isoprene synthase was engineered for enhanced catalytic efficiency and thermostability, and also enhanced methane-to-isoprene conversion when applied to methanotrophs, highlighting its potential for methane-based biomanufacturing.