Selector adeno-associated viral vectors facilitate on-target precise genome editing and purge off-target chromosomal insertions

This study establishes selector adeno-associated viral (AAV) vectors for the targeted and precise installation of chromosomal gene-sized edits. The incorporation of a marker-free co-selection system based on a cheap small molecule permits selector AAV vectors to, next to fostering targeted DNA editing, purge off-target chromosomal insertions, improving the quality of engineered cell products.