In vivo continuous evolution via phenotypic sorting to alleviate metabolic bottlenecks in β-alanine production

This study uses a T7 RNA polymerase-deaminase fusion editor-based in vivo evolution platform, coupled with a biosensor for simultaneous mutagenesis and screening, to enhance the activity of the rate-limiting enzyme in β-alanine biosynthesis, thereby offering a scalable strategy for optimizing microbial cell factories.