Engineering Halomonas for low-salt, open, and unsterile production of polyhydroxybutyrate

Wild-type Halomonas bluephagenesis TD1.0 was engineered to generate an iterative SSM series highly adapted to low-salinity conditions. The multiple strategy approach included genetic engineering, adaptive evolution, production module, and process engineering. Together, these synergistic strategies enabled the construction of broadly salinity-adaptive Halomonas chassis suitable for low-salinity, open, and nonsterile biomanufacturing.