Development of a high-efficiency N-acetylneuraminic acid production platform through multi-pathway synergistic engineering

This study leverages artificial intelligence (AI)-driven enzyme mining and metabolic reprogramming for precise precursor control, alongside dual-carbon source synergy, establishing a cost-effective N-acetylneuraminic acid (NeuAc) biomanufacturing platform. This systematic synthetic biotechnology framework not only elevates NeuAc yields, but also offers generalizable solutions to persistent bottlenecks in microbial-based bioproduction.