Connector-enabled integration of Golden Gate Assembly and yeast recombination for streamlined multigene pathway construction in the biofoundry workflow

Microbial bioconversion is a key driver in the modern biotechnology industry, enabling sustainable production of foods, chemicals, fuels, and nutraceuticals [1–3]. Central to this capability is metabolic engineering, which strategically rewires microbial metabolism to synthesize value-added bioproducts [4]. Achieving high-yield production of molecules of interest in microbial hosts often requires introducing heterologous metabolic pathways, enhancing precursor supply, eliminating competing rea