Biosensor-guided indexing of genome evolution for boosting microbial chemical synthesis

This study developed a biosensor-driven genome evolution (BRIDGE) workflow that enables marker-free engineering of microbial hosts. As an example, protocatechuic acid (PCA) overproduction in Pseudomonas putida was achieved by BRIDGE. Considering the great variety of available biosensors, BRIDGE may represent a generalizable framework for biosensor-driven evolution of microbial cell factories.