Slow-binding inhibition of cholinesterases

Reversible inhibition of an enzyme, an activity in which the inhibiting molecular entity (often a small chemical called ligand or inhibitor) associates and dissociates from the protein's binding site, is a very fast process. During enzyme-substrate interaction, enzyme-inhibitor equilibrium is established within microseconds. Thus, classical reversible inhibitors are characterized by rapid on/off rates. However, a number of enzymes do not respond instantly to reversible inhibitors. In such cases,