New methods of enhancing efficiency of genetic engineering in mice and rats

A group of researchers led by Tomoji Mashimo, Associate Professor, Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University and Kazuto Yoshitomi, Assistant Professor, Mouse Genomics Resource Laboratory, National Institute of Genetics, Research Organization of Information and Systems developed two new gene modification methods: lsODN (long single-stranded oligodeoxynucleotide) and 2H2OP (two-hit two-oligo with plasmid). These methods use CRISPR (Clustered Regularly