Genome editing: Efficient CRISPR experiments in mouse cells

In order to use the CRISPR-Cas9 system to cut genes, researchers must design an RNA sequence that matches the DNA of the target gene. Most genes have hundreds of such sequences, with varying activity and uniqueness in the genome. The search for the best sequences is therefore hardly achievable by hand. The new "CrispRGold" program helps scientists to identify the most effective and specific RNA sequences. It has been devised by a group of researchers headed by Prof. Klaus Rajewsky of the Max Del