Parallelized CRISPR-Cas9 Generates Sequence Variants Genome Wide

Let’s say you want to learn how a particular DNA sequence variant affects different genes. You could painstakingly introduce the variant, and monitor its effect, gene by gene. How tedious. Alternatively, you could adapt a technique that has been used to assess genetic knockdowns genome wide. This technique is a library-based technique in which many RNAs are used to guide the DNA-snip-inducing Cas9 molecule to its gene targets.To date, the technique has been used to introduce gene-inactivat