Nonviral CRISPR Technology Developed for Faster, Cheaper T-Cell Engineering

Researchers at the University of California, San Francisco (UCSF) have developed what they describe as a fast, simple CRISPR -based method for genetically reprogramming T cell function, without using viral vectors. The technique, developed in the laboratory of associate professor of microbiology and immunology Alex Marson, M.D., Ph.D., uses electroporation to permeabilize T cells to the CRISPR gene editing components and DNA, and can be used to insert even large gene sequences at specified, CRIS