DNA Crosslink Repair Central to CRISPR Insert Success

In CRISPR-Cas9 gene editing, the balance between two DNA repair pathways gets all the attention. Everybody wants to know, when Cas9 makes a cut, will DNA be repaired by non-homologous end joining (NHEJ), a quick-and-dirty procedure, or by homology-directed repair (HDR), a relatively painstaking procedure guided by template DNA. Well, it turns out that a third pathway can tilt the balance toward NHEJ or HDR, influencing the success rates of gene editing applications, including those with therapeu