CRISPR Screens Stem Cell Genome, Charts Essentialome

Scientists at the Hebrew University of Jerusalem have generated an atlas of genes—the essentialome —that are essential for the normal growth and maintenance of human pluripotentent stem cells (hPSCs). The team, led by Nissim Benvenisty, M.D., Ph.D., director of the Azrieli Center for Stem Cells and Genetic Research and the Herbert Cohn Chair in Cancer Research, carried out a genome-wide CRISPR-Cas9 loss-of-function screen on haploid human embryonic stem cell (hESCs) to highlight whic