CRISPR-AID Pulls Off a Metabolic Engineering Trifecta

Activation. Interference. Deletion. Together, they spell AID, and they are being combined so that they may better aid metabolic engineering. AID refers to three genetic manipulations that can be accomplished simultaneously by a newly developed CRISPR system.Ordinarily, these manipulations are carried out sequentially, so that their combined effects emerge only after a laborious multistep process is completed. CRISPR-AID, however, promises to expedite metabolic engineering projects in which multi